While (whilst?) organizing one of my old e-mail accounts last night, I ran across a page that I had intended to use on my old U of A website but never put up. I present it here in its entirety (except for the so-called links, which I obviously never got around to putting in) and encourage everyone to try it. (Note: don’t judge the cocktail recipes too harshly. I was 17 when I did this page and I was dying to be old enough to drink.) (Note 2: I also actually did do it with an onion, and I really don’t recommend it. But all the bar equipment works well enough.)
There may be pictures later - I’m going to do it this Sunday, because I already think I’m going to be stood up again.
“The Egg-White of Life”
As you go through this tutorial, you can click on the links to see the background science behind each of the steps. Warning : these explanations are slightly more than slightly bio-weenie friendly, so in case of confusion (i.e. not being a genetics undergrad), my advice is as follows: hide in a corner and weep.
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Materials
Equipment and glassware - can substitute similar-shaped containers if needed!
Shot glass
Measuring spoons (or teaspoons and a really good eye)
Two or three small, preferably glass bowls
Garlic press, grater, blender, or huge man with scimitars
Champagne flute
Coffee filter
Glass cocktail swizzle stick
Small vial
Reagents - attempt not to substitute!
Ice!
Distilled (or deionized, bottled) water
Salt
Baking soda
Dishwashing detergent (or cheap shampoo)
A fruit or a vegetable, eg. banana, cauliflower, your brother
Isopropyl alcohol (highest percentage possible!)
Step 1: Making the buffer.
Measure about 1/4 tsp of baking soda and about 1 tsp dishwashing liquid into the clean glass bowl. Then add 4oz of distilled water on top of that. (This is where the markings on the shot glass come in handy. Also, the shot glass will subsequently be so clean that you can mix yourself a shot in it. I recommend a Fallen Angel: 1 oz Sambuca, on which you gently float 1/2 oz bourbon. Down in one gulp. Weep for lost innocence.)
Chill this buffer very thoroughly on icewater or in your fridge. I also recommend keeping instruments in an icewater bath when not in use.
Step 2: Homogenizing the source tissue.
Mush your fruit or vegetable (I did it with an onion: not recommended at all) in a food processor with about 1 oz of water, pulsing in 10-second bursts; or use a garlic press on the bastard if you haven’t got the patience. (I never tried extracting DNA from mint, but you may as well use the garlic press while you have it out, right? Have a Mint Julep to reward yourself for getting this far: Crush 2 sprigs of mint and put them in a julep cup or highball glass. Add 2 tsp water and 1 tsp superfine or confectioners sugar. Fill to top with crushed ice. Top off with 3 oz bourbon, if your shot glass is clean enough. If not, drink from the bottle and eat the ice and mint with a spoon.)
Chill your fruit/vegetable mush too, while you get the rest of your experiment ready.
Step 3: Separation of DNA and plant matter.
Put about 1 tsp of the cold vegetable puree into a clean container and mix in about 1 tbsp of your buffer. Don’t be tempted to use more buffer or puree: it really just slows things down. Stir vigorously for at least two minutes. You can even cover it with plastic wrap (tight, mind you) and shake your booty for about that long. (If picking the DNA out of vegetables doesn’t interest you any more, have a Bloody Maria to take your mind off it: mix 2 oz tequila, 4 oz tomato juice - hope you kept your shot glass clean - half a lemon’s worth of juice, 1/2 tsp horseradish, dash of worcestershire sauce, dash of tabasco sauce, and whatever else you feel like throwing in a clean highball glass; strain over ice cubes and drink in Mexican bar.)
Once you’re done shaking it, chill the mix again.
Separating the DNA from the visible plant matter is a tricky business. If you don’t have a centrifuge at home, fold a coffee-filter into a cone and poke (don’t cut) a very small hole in the bottom. Set it in your champagne flute and tape into place. Pour the chilled mix into the filter, then sit back and wait. This tends to take a very long time due to the viscosity of the mixture, but again, do not use extra buffer. (While you’ve got the time, and the coffee-filter packet out, may as well make some Irish Coffee, Buena Vista style: Heat a glass mug with boiling water, dump it out - but not into your DNA - add about 3 sugar cubes to the hot glass, 1 oz Irish Whiskey, and strong black coffee to about 2/3rds full, then pour cold cream over a spoon to the top of the glass.)
While you’re letting the stuff drip through, or finishing off your Irish Coffee, chill some isopropyl alcohol in the freezer until it is ice cold (don’t worry, it won’t freeze).
Step 4: Extracting the DNA from the liquidy stuff.
This is the really ‘fun’ part!
Hopefully the liquidy gook will not have filled more than an inch or so of your champagne flute (or other suitable skinny vessel). At this point you can toss out the coffee filter with the leftover vegetable stuff on it, or alternatively, put it in another Bloody Soapy Maria.
Carefully dribble cold isopropyl alcohol onto the surface of the liquidy stuff left in the flute. This is easiest if you tilt the flute slightly and pour along the inner surface of the glass.
Now for the cool part - collecting a snotball composed of the chemical of life. Slide your glass swizzle stick into the interface between the liquidy stuff and the alcohol, and gently move it up and down through the layers. You should see some cloudy, egg-whitey stuff sticking to the, uh, stick. Transfer this carefully into your clean vial by scraping along the edge and repeat until there are only cobwebby pieces in the alcohol. (And if seeing DNA makes you randy for egg-white, have a Classic Fizz: shake 3 oz dry gin, half a lemon’s juice, 1 egg white, 1 tbsp heavy cream, 1 tbsp sugar, 1 tsp orange flower water, and some ice together until your arms get tired. Then shake for another ten minutes. Strain over ice into a large glass and top with tonic water. Stir and, if you can find your mouth, drink.)
Step 5: DNA Party with Your Friends!
Invite all your friends over and show them your cool DNA in a vial. Then have everybody pick a fruit or vegetable and do it all over again! Woohoo!